shrna oligonucleotide sequences Search Results


shrna oligonucleotide sequences targeting mouse irf8 gene mrna  (SunBio Inc)
90
SunBio Inc shrna oligonucleotide sequences targeting mouse irf8 gene mrna
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Shrna Oligonucleotide Sequences Targeting Mouse Irf8 Gene Mrna, supplied by SunBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pmc05090347-236-5-12?v=SunBio+Inc
Average 90 stars, based on 1 article reviews
shrna oligonucleotide sequences targeting mouse irf8 gene mrna - by Bioz Stars, 2026-07
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oligonucleotides corresponding to prdm4- and pten-specific small hairpin rna (shrna) sequences  (Shanghai GenePharma)
90
Shanghai GenePharma oligonucleotides corresponding to prdm4- and pten-specific small hairpin rna (shrna) sequences
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Oligonucleotides Corresponding To Prdm4 And Pten Specific Small Hairpin Rna (Shrna) Sequences, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pmc08102194__41388_2021_1765_MOESM2_ESM-21-0-15?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
oligonucleotides corresponding to prdm4- and pten-specific small hairpin rna (shrna) sequences - by Bioz Stars, 2026-07
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oligonucleotide probes for construction of shrna targeting rar reverse sequence  (Microsynth ag)
90
Microsynth ag oligonucleotide probes for construction of shrna targeting rar reverse sequence
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Oligonucleotide Probes For Construction Of Shrna Targeting Rar Reverse Sequence, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pm19179438-85-0-21?v=Microsynth+ag
Average 90 stars, based on 1 article reviews
oligonucleotide probes for construction of shrna targeting rar reverse sequence - by Bioz Stars, 2026-07
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kif18b-targeting short hairpin rna (shrna) oligonucleotide sequences  (GenScript corporation)
90
GenScript corporation kif18b-targeting short hairpin rna (shrna) oligonucleotide sequences
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Kif18b Targeting Short Hairpin Rna (Shrna) Oligonucleotide Sequences, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pm35251343-42-1-17?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
kif18b-targeting short hairpin rna (shrna) oligonucleotide sequences - by Bioz Stars, 2026-07
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shrna oligonucleotides were derived from the murine gro-a sequence (nih genbank accession no. j04596)  (Becton Dickinson)
90
Becton Dickinson shrna oligonucleotides were derived from the murine gro-a sequence (nih genbank accession no. j04596)
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Shrna Oligonucleotides Were Derived From The Murine Gro A Sequence (Nih Genbank Accession No. J04596), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pm16618733-99-35-53?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
shrna oligonucleotides were derived from the murine gro-a sequence (nih genbank accession no. j04596) - by Bioz Stars, 2026-07
90/100 stars
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shrna oligonucleotide sequences of lincrna-ror  (Shanghai GenePharma)
90
Shanghai GenePharma shrna oligonucleotide sequences of lincrna-ror
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Shrna Oligonucleotide Sequences Of Lincrna Ror, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pm27696511-92-7-19?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
shrna oligonucleotide sequences of lincrna-ror - by Bioz Stars, 2026-07
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oligonucleotide sequences of lincrna-ror-specific shrna and nontargeting shrna  (Shanghai GenePharma)
90
Shanghai GenePharma oligonucleotide sequences of lincrna-ror-specific shrna and nontargeting shrna
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Oligonucleotide Sequences Of Lincrna Ror Specific Shrna And Nontargeting Shrna, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pm25651893-36-7-23?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
oligonucleotide sequences of lincrna-ror-specific shrna and nontargeting shrna - by Bioz Stars, 2026-07
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lentiviruses harboring the short hairpin rna (shrna) target oligonucleotides for sequence mock  (Ribobio co)
90
Ribobio co lentiviruses harboring the short hairpin rna (shrna) target oligonucleotides for sequence mock
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Lentiviruses Harboring The Short Hairpin Rna (Shrna) Target Oligonucleotides For Sequence Mock, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pm36952219-54-0-19?v=Ribobio+co
Average 90 stars, based on 1 article reviews
lentiviruses harboring the short hairpin rna (shrna) target oligonucleotides for sequence mock - by Bioz Stars, 2026-07
90/100 stars
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shrna oligonucleotide sequence  (BGI Shenzhen)
90
BGI Shenzhen shrna oligonucleotide sequence
( a ) The effect of AGEs on <t>IRF8</t> was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative <t>mRNA</t> levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.
Shrna Oligonucleotide Sequence, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+oligonucleotide+sequences/pm29663367-52-14-37?v=BGI+Shenzhen
Average 90 stars, based on 1 article reviews
shrna oligonucleotide sequence - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


( a ) The effect of AGEs on IRF8 was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative mRNA levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.

Journal: Scientific Reports

Article Title: AGEs Induced Autophagy Impairs Cutaneous Wound Healing via Stimulating Macrophage Polarization to M1 in Diabetes

doi: 10.1038/srep36416

Figure Lengend Snippet: ( a ) The effect of AGEs on IRF8 was analyzed. The RAW264.7 cells were treated with or without AGEs for 24 h, CQ (50 nM) was added simultaneously for 24 h to inhibit autophagic flux. Western blotting was performed to demonstrate IRF8 protein bands. ( b ) The RAW264.7 cells, infected with pLVT 7 (control shRNA) or pLVT 1533 (shIRF8), were treated with and without AGEs (100 μg/ml) for 24 h. Western blotting was performed to demonstrate IRF8 and LC3 protein bands. ( c ) The infected cells were incubated simultaneously with AGEs for 24 h, immunofluorescence was employed for LC3 staining. The positive cells (red) are presented, blue staining represents nucleus. ( d ) The infected cells were stimulated by LPS for M1 polarization with and without AGEs treatment for 24 h. Relative mRNA levels were detected using real-time PCR (n = 3). Data is presented as the mean ± SD.* P < 0.05, ** P < 0.01.

Article Snippet: shRNA oligonucleotide sequences for targeting mouse IRF8 gene mRNA was designed by Sunbio Medical Biotechnology (Shanghai, China).

Techniques: Western Blot, Infection, shRNA, Incubation, Immunofluorescence, Staining, Real-time Polymerase Chain Reaction

AGEs up-regulate expression of IRF8 and induce macrophage autophagy. Enhanced autophagic activity promotes macrophage polarization to M1. Furthermore, increased M1 macrophages result in excessive inflammation, which leads to impaired wound healing. Together, the mechanism of IRF8-autophagy-M1 polarization is critical for AGEs-induced autophagy impaired wound healing in diabetes.

Journal: Scientific Reports

Article Title: AGEs Induced Autophagy Impairs Cutaneous Wound Healing via Stimulating Macrophage Polarization to M1 in Diabetes

doi: 10.1038/srep36416

Figure Lengend Snippet: AGEs up-regulate expression of IRF8 and induce macrophage autophagy. Enhanced autophagic activity promotes macrophage polarization to M1. Furthermore, increased M1 macrophages result in excessive inflammation, which leads to impaired wound healing. Together, the mechanism of IRF8-autophagy-M1 polarization is critical for AGEs-induced autophagy impaired wound healing in diabetes.

Article Snippet: shRNA oligonucleotide sequences for targeting mouse IRF8 gene mRNA was designed by Sunbio Medical Biotechnology (Shanghai, China).

Techniques: Expressing, Activity Assay